Inicio >  Term: biotinylated-DNA
biotinylated-DNA

A DNA molecule labelled with biotin by incorporation of biotinylated-dUTP into a DNA molecule. It is used as a non-radioactive probe in hybridization experiments, such as Southern transfer. The detection of the labelled DNA is achieved by complexing it with streptavidin (an antibiotic with a high affinity for biotin) to which is attached a colour-generating agent such as horseradish peroxidase that gives a fluorescent green colour upon reaction with various organic reagents.

0 0

Creador

  • bluemoon
  •  (Gold) 2587 puntos
  • 100% positive feedback
© 2024 CSOFT International, Ltd.