Inicio > Term: biotinylated-DNA
biotinylated-DNA
A DNA molecule labelled with biotin by incorporation of biotinylated-dUTP into a DNA molecule. It is used as a non-radioactive probe in hybridization experiments, such as Southern transfer. The detection of the labelled DNA is achieved by complexing it with streptavidin (an antibiotic with a high affinity for biotin) to which is attached a colour-generating agent such as horseradish peroxidase that gives a fluorescent green colour upon reaction with various organic reagents.
- Parte del discurso: noun
- Industria/ámbito: Biotecnología
- Categoría: Genetic engineering
- Organization: FAO
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- bluemoon
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