The Twinrova Sisters are a pair of Gerudo witches who play an important role in a few games. They are the surrogate mothers of the Gerudo King, Ganondorf, being his more devoted servants. They can brainwash others to serve Ganondorf (they do so to the Sage Nabooru in the Spirit Temple of Ocarina of Time) and merge to form the stronger witch Twinrova. The brooms the sisters use to fly become scepters through which Twinrova channels her power.

They portray the same characters in Majora's Mask, though they are more benevolent than their Ocarina of Time counterparts. Koume operates a tour-boat service in the Southern Swamp, while Kotake runs a potion store in the same region. At one point, Koume is injured by the Skull Kid in the nearby Lost Woods and requests that Link bring her a red potion from Kotake's store to heal her wounds.