Anju first appears in Ocarina of Time but is unnamed and generally referred to as the "Cucco Lady." She resides in Impa's old house in Kakariko Village. She says that she is allergic to Cuccos, and hers are always escaping from their pen. The first time that Link rounds them up for her, he receives an empty bottle in return.

Majora's Mask is the first of the Zelda series in which Anju is named. She is troubled over the disappearance of her fiancé, Kafei. In a lengthy side-quest resulting in the reunion of those betrothed, Link can acquire several masks: the Keaton Mask, the Kafei Mask, the Postman's Hat, and the Couple's Mask.

Anju reappears in The Minish Cap, wherein she again employs Link's help to retrieve her Cuccos, compensating with rupees and a Piece of Heart.