A type of in vitro (outside the body) fertilization that is used principally to overcome male infertility. Intracytoplasmic sperm injection (ICSI) involves injecting a single sperm into an egg that has been removed from a woman’s ovary. The fertilized egg can then be returned to the woman's uterus. Sperm washing combined with ICSI can be considered as a reproductive option for an HIV discordant couple. ICSI is considered when the man is the HIV-infected partner and insemination with donor sperm from an HIV-uninfected male is not an option.